![]() Here we fill this gap and describe AScall – the interactive web application for the proposed genotyping method. Earlier, we have confirmed and published the AS-qPCR method, but standard software for qPCR analysis does not suit AS-qPCR data. The new approach allows for multiplexing up to 480 reactions per 96 well plate and differs from common qPCR based genotyping methods. To determine the genetic mismatches between a donor and a recipient, we have implemented a real-time PCR method in conjunction with allele-specific primers (AS-qPCR). MiHAs originate from donor-recipient discrepancies in single nucleotide polymorphisms, insertions, and deletions. They may cause both beneficial and detrimental effects in forms of graft-versus-tumor and graft-versus-host accordingly. Minor histocompatibility antigens (MiHA) are critical elements for the immune response after allogeneic hematopoietic stem cell transplantation. ![]() ![]()
0 Comments
Leave a Reply. |
Details
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |